Discovery of a rare human gene mutation that causes MAIT cells to disappear

A collaboration between Monash Health, the Australian Genomics Health Alliance (AGHA) and researchers at the Monash Biomedicine Discovery Institute has led to the discovery of a rare single gene mutation in a patient that eliminates an immune cell population, namely MAIT cells.

This study’s journey began with a patient of Dr Samar Ojaimi from Monash Health who presented with a mild primary immunodeficiency with no known cause. He was identified as a candidate for the Genetic Immunology Flagship of AGHA led by Professor Matthew Cook from the Centre for Personalised Immunology at the Australian National University, which focuses on identifying genetic causes for immunological diseases.

The genome sequencing by the AGHA team identified a rare mutation in the gene encoding a protein called MR1, which normally helps initiate an inflammatory response from an immune cell population called mucosal-associated invariant T (MAIT) cells.  However, upon further investigation by a research team from Monash BDI, led by Dr Lauren Howson, they uncovered there had been a complete loss of this immune cell population, while the rest of the immune system remained intact.

“We studied the patient’s MR1 protein structure and found that the mutation prevented MR1 from being able to bind the vitamin metabolite it normally presents in order to activate MAIT cells. This led us to look at the patient’s immune system to see what effect the mutation had on the MAIT cell population and we were surprised to find it completely gone,” Dr Howson said.

A man gazes at the complex protein structures of his own immune system, and the rare genetic mutations that caused his illness, leading scientists to a dramatic discovery. Image © Dr Erica Tandori

Published in Science Immunology, the study demonstrates the power of interdisciplinary collaboration to uncover the impact of a single gene mutation and aid in diagnosis of rare immune disorders.

It not only advances the MR1 and MAIT cell biology research fields, but also demonstrates the substantial impact that discovery-based research can have when combined with clinical and genetic research, creating an avenue for advanced personalised medicine for rare genetic and immune disorders.

“This occurrence of a single immune cell population loss in a person gives us invaluable insight into the important role that this cell type plays in human immune responses,” Dr Howson said.

Professor Cook said: “Human genomics is a powerful method for advancing our understanding of the complexity of immunity.

“Genome sequencing has emerged as a crucial tool for both diagnosis and discovery of immune-mediated disease.”

Image: A man gazes at the complex protein structures of his own immune system, and the rare genetic mutations that caused his illness, leading scientists to a dramatic discovery. Artwork by Dr Erica Tandori.

Read the paper titled: “Absence of mucosal-associated invariant T cells in a person with a homozygous point mutation in MR1” Science Immunology.

Original article

Calling all MAITs: teaming up to solve the intricacies of the immune system

Specialised immune cells, called MAIT cells, receive activation signals from the immune system to fight bacteria. Without the right signals and guidance, they can contribute to cancer and autoimmune diseases.

Little was known about how signals were provided to MAIT cells, until now. Australian-based researchers have zoomed in on the molecular intricacies of the ‘go’ signal to learn how it is initiated and how we can boost it for therapeutic purposes.

MAIT cells sit in common sites of infection, primed to rapidly combat invading bacteria and yeast by recognising the presence of products made along the way when bacteria produce vitamin B.

Humans are not able to produce vitamin B, instead sourcing it from our diet. The presence of the building blocks of vitamin B in the body therefore signify the presence of bacteria and/or yeast as they must be coming from them rather than our own cells.

A protein called MR1 captures molecules that are building blocks for vitamin B2 (riboflavin) or B9 (folic acid), and other small molecules to hold them up as beacons for MAIT cells to detect – MAIT cells would not interact with them otherwise.

If a MAIT cell interacts with MR1 and riboflavin compound, it becomes an activated fighter for the immune system, but if a folic acid compound is captured by MR1 instead, most MAIT cells will not see it and do nothing (as though it were covered in an invisibility cloak).

It was this fine-line between whether or not a MAIT cell can see particular small compounds, thereby driving MAIT cell activation, that the researchers wanted to investigate.

Previous work by a research collaboration spanning the Doherty Institute, Monash University and University of Queensland revealed vitamin B as the trigger for MAIT cell activation.

Building on this foundation, the same research groups aimed to determine the rules that govern this activation process. They developed a suite of riboflavin derivatives to find one that provides the best response, potentially for use in therapy, in a study published today in Nature Immunology.

“Our aim was to learn how much the components of the antigen move around in solution and which ones are critical for sandwiching between the two target proteins to bind then activate the MAIT immune cell,” says Professor David Fairlie, a senior researcher on the study.

Miss Geraldine Ler, Dr Weijun Xu and colleagues in Professor David Fairlie’s research group at the University of Queensland synthesised and characterised a suite of small molecules as chemical variants of the riboflavin-based molecules that activate MAIT cells. Dr Alexandra Corbett and colleagues at the Doherty Institute then tested their ability to bind MR1 and activate MAIT cells. Dr Wael Awad and Professor Jamie Rossjohn at the Monash Biomedicine Discovery Institute zoomed in on the molecular interactions between MAIT cells, MR1 and the vitamin B derivatives.

“When MR1 captures vitamin B derivatives, they are mostly buried so that MAIT cells can only see a minute section that pokes out. It is therefore surprising that such a small part can drive either activation or inhibition, so we wanted to look a little closer,” says Dr Corbett, another senior researcher on the study.

“We investigated three essential properties of each compound: how long they last before breaking down, their ability to coax cells to put more MR1 on their surface so that they can call on more MAIT cells, and how the MAIT cells can see these MR1-‘go’ signals, resulting in their activation. We were able to discover the rules that govern MAIT cell activation, how MAIT cells discriminate between different vitamin B derivatives, and how we can better design potent targets,” she said.

“Using advanced imaging tools, we unearthed the molecular principles underpinning how MR1 captures and presents these small molecules to MAIT receptors triggering the ‘go’ signals,” Dr Awad, first author on the study, said.

“Here, a focused network of nano-connections termed ‘MAIT Interaction Triad’ fine-tunes this process between MR1, metabolite, and MAIT receptor. These discoveries could pave the way for development of novel T cell therapy,” he said.

“This study demonstrates the power of collaboration and the insights we can gain with inter-disciplinary science,” Dr Corbett said.

The more that is understood about MAIT cells and the nuances that boost or prevent their activation, the better they can be manipulated in the context of disease.

Amplifying MAIT cell responses to invading bacteria may help to control infections. In other contexts, however, MAIT cells can be destructive, such as in chronic infections and cancer. Being able to enhance or block them by controlling the visibility of riboflavin compounds and the “go” signals may therefore be highly beneficial for infection, cancer, inflammatory bowel disease, and a range of other diseases.

With this new insight, the teams of Doherty Institute, Monash University and University of Queensland researchers can now design drugs that either activate or block MAIT cells as new immunotherapy targets; potentially for a multitude of diseases.

Read the full paper in Nature Immunology titled The molecular basis underpinning the potency and specificity of MAIT cell antigens.

Nature Immunology cover image 

Original article